We focus on identification and functional analysis of signaling pathways, transcriptional and epigenetic mechanisms controlling gene expression in microglia and glioma tumor cells. Employing chromatin immunoprecipitation (ChIP), transcriptomics, next generation sequencing (NGS), and bioinformatics, we try to understand transcriptional changes in microenvironment that promote glioma development. Strong collaboration with hospitals and use of advanced experimental models allows us to study potential glioma therapeutic strategies that can be employed in the clinic.

Our facilities include: transcriptomics, genome wide chromatin immunoprecipitation, next generation sequencing, data mining services for transcriptomics, genomics and proteomics.

Techniques mastered in LMN

  • cellular/animal models of neuroinflammation, tumor-host interactions and depression

  • patient-derived cancer stem cells cultures

  • sorting immune cells from tumor tissues

  • chromatin immunoprecipitation (ChIP)

  • transcriptomic microarray analysis

  • scRNA-seq and DNA next generation sequencing

  • cloning and production of cells expressing shRNA or recombinant proteins

  • in vivo live fluorescence and bioluminescence imaging (Xtreme II system)